Integrating life cycle assessment and characterisation techniques: A case study of biodiesel production utilising waste Prunus Armeniaca seeds (PAS) and a novel catalyst.

Prunus Armeniaca seed (PAS) oil was utilised as a waste biomass feedstock for biodiesel production via a novel catalytic system (SrO-La2O3) based on different stoichiometric ratios. The catalysts have been characterised and followed by a parametric analysis to optimise catalyst results. The catalyst with a stoichiometric ratio of Sr: La-8 (Sr-La-C) using parametric analysis showed an optimum yield of methyl esters is 97.28% at 65 °C, reaction time 75 min, catalyst loading 3 wt% and methanol to oil molar ratio of 9. The optimum catalyst was tested using various oil feedstocks such as waste cooking oil, sunflower oil, PAS oil, date seed oil and animal fat. The life cycle assessment was performed to evaluate the environmental impacts of biodiesel production utilising waste PAS, considering 1000 kg of biodiesel produced as 1 functional unit. The recorded results showed the cumulative abiotic depletion of fossil resources over the entire biodiesel production process as 22,920 MJ, global warming potential as 1150 kg CO2 equivalent, acidification potential as 4.89 kg SO2 equivalent and eutrophication potential as 0.2 kg PO43- equivalent for 1 tonne (1000 kg) of biodiesel produced. Furthermore, the energy ratio (measured as output energy divided by input energy) for the entire production process was 1.97. These results demonstrated that biodiesel obtained from the valorisation of waste PAS provides a suitable alternative to fossil fuels.

Various potential techniques to reduce the water footprint of microalgal biomass production for biofuel-A review.

Due to their rapid growth rates, high lipid productivity, and ability to synthesize value-added products, microalgae are considered as the potential biofuel feedstocks. However, among the several bottlenecks that are hindering the commercialization of microalgal biofuel synthesis, the issue of high water consumption is the least explored. This analysis, therefore, examines the factors that decide water use for the production of microalgae biofuel. Microalgae biodiesel water footprint varies from 3.5 to 3726 kg of water per kg of biodiesel. The study further investigates the cause for large variability in the estimation of the water footprint for microalgae fuel. Various strategies, including the reuse of harvested water, the use of high density cultivation that could be adopted for low water consumption in microalgal biofuel production are discussed. Specifically, the review identified a reciprocal relationship between biomass productivity and water footprint. On the basis of which the review emphasizes the significance of high density cultivation, which can be inexpensive and feasible relative to other water-saving techniques. With the setback of water scarcity due to the rapid industrialization in developing countries, the implementation of the cultivation system with a focus on minimizing the water consumption is inevitable for a successful large scale microalgal biofuel production.

Biodiesel production over a catalyst prepared from biomass-derived waste date pits.

Date palms are predominately produced in arid regions and the date pits, or seeds, produced from them are sometimes considered to be a waste. Date pits, ground to powder following an oil extraction, were used to synthesize a renewable heterogeneous catalyst. The green carbon catalyst was modified by an alkaline earth metal oxide (CaO). The oil extracted from date pits was transformed into biodiesel. The biodiesel process was optimized and the optimal yield was 98.2 wt% at a reaction temperature of 70 °C, reaction time ∼120 min, methanol to oil molar ratio of 12 and catalyst loading of 4.5 wt%. The quality of the produced biodiesel meets the standard limits set by regulating agencies (ASTM, EU) which indicates its suitability to be used as a fuel. Thus, it can be concluded that the green carbon catalyst synthesized from waste date pits has a high potential for biodiesel production.

Cyanobacteria as Chassis for Industrial Biotechnology: Progress and Prospects.

Cyanobacteria hold significant potential as industrial biotechnology (IB) platforms for the production of a wide variety of bio-products ranging from biofuels such as hydrogen, alcohols and isoprenoids, to high-value bioactive and recombinant proteins. Underpinning this technology, are the recent advances in cyanobacterial "omics" research, the development of improved genetic engineering tools for key species, and the emerging field of cyanobacterial synthetic biology. These approaches enabled the development of elaborate metabolic engineering programs aimed at creating designer strains tailored for different IB applications. In this review, we provide an overview of the current status of the fields of cyanobacterial omics and genetic engineering with specific focus on the current molecular tools and technologies that have been developed in the past five years. The paper concludes by giving insights on future commercial applications of cyanobacteria and highlights the challenges that need to be addressed in order to make cyanobacterial industrial biotechnology more feasible in the near future.